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First detection of bee viruses in hoverfly (syrphid) pollinators: Supporting data - accepted subject to minor revision in Biology Letters (2018)

dataset
posted on 26.01.2018 by Emily Bailes, Kaitlin R Deutsch, Judy Bagi, Lucila Rondissone, Mark Brown, Owen T Lewis
Supporting data for "First detection of bee viruses in hoverfly (syrphid) pollinators" (accepted subject to minor revisions in Biology Letters, 2018)

Bailesetal_HoverflyScreening.xlsx
Information on the samples screened for viruses and the viruses detected and the NCBI sequences relating to individual samples

Bailesetal_qRTPCR.xlsx

qRT-PCR data for SBV and BQCV titres, including Ct values, in honey bees and hoverflies


Bailesetal code.R
R code used for analysing differences in viral titres and viral presence/absence between honey bees and hoverflies

Bailesetal_SBV-clustal-alignment.txt
Alignment of SBV sequences from Clustal Omega

Bailesetal_BQCV-clustal-alignment.txt
Alignment of BQCV sequences from Clustal Omega

.abi files
Sequences traces are named SeqID_Virus_SampleID.abi - NVBI seqeuence IDs that relate to the sequencing trace are given in Bailesetal_HoverflyScreening.xlsx
- The higher quality 3' end of S1_SBV_Am7_2_3primeend.ab1
was used to extend the sequence of S15_SBV_Am7_2.ab1
- Sequence B9B_BQCV_Ea20_pGEM.ab1 is cloned into pGEM and sequenced from the M13F site, it is in reverse orientation.





Funding

John Fell OUP Research Fund grant 152/061, BBSRC grant BB/N000668/1

History

Date Available

25/01/2018

File Formats

.txt .xlxs .R .abi

Licence

Exports